Part:BBa_K801098:Design

O-methyltransferase 1 (OMT1) coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 479
  Illegal XhoI site found at 294
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

http://www.ncbi.nlm.nih.gov/nucleotide/167613934/

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, aromatic prenyltransferase

Abbreviations: OMT = O-methyltransferase

Design Notes

Related BioBrick:

Other versions: BBa_K801097: OMT1 + yeast consensus sequence

Related BioBricks:

• BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence
• BBa_K801091: phenylalanine ammonia lyase (PAL) coding region
• BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
• BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region
• BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
• BBa_K801095: Naringenin - chalcone synthase (CHS) coding region
• BBa_K801096: Aromatic prenyltransferase (APT) coding region
• BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: O-methyltransferase
• Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: EC 2.1.1

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid with OMT provided by Jonathan E. Page, National Research Council Canada, Plant Biotechnology Institute, Canada

Organism:
Genesequence derived from Humulus lupulus

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/18223037 Pubmed : Nagel, J., Culley, L.K., Lu, Y., Liu, E., Matthews, P.D., Stevens, J.F., Page, J.E., 2008. EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol. Plant Cell 20, 186–200]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/EU309725.1 GenBank: Humulus lupulus O-methyltransferase 1 (OMT1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/B0ZB55 Uniprot: O-methyltransferase 1]